Composite

Part:BBa_K1725101

Designed by: Mhairi Davidson   Group: iGEM15_Glasgow   (2015-09-10)

PhlF repressible promoter + weak RBS + SrpR repressor + terminator + SrpR repressible promoter + wea

.PhlF repressible promoter drives the expression of srpr repressor with B0032 RBS and B0010+ terminator and SrpR repressible promoter drives the expression of phlf repressor with B0032 RBS and B0010+ terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 854
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 565
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None